Fig. 5

Actr10 functions cell autonomously in axons.

(A–C) Expression of actr10 mRNA was assayed using in situ hybridization with DIG-labeled riboprobes and alkaline phosphatase-mediated NBT/BCIP precipitation. actr10 is ubiquitously expressed at all time points tested, with elevated expression in the nervous system at later stages (3 and 5 dpf). Arrows point to the pLL ganglion in B and C. (D) Neuronal mRFP-Actr10 expression does not alter axon morphology or mitochondrial localization in wildtype larvae at 4 dpf. Mitochondria were visualized by Cytochrome c immunolabeling in NM3 axon terminals. (E) Uninjected actr10 mutants have high levels of Cytochrome c immunolabling in NM3 axon terminals at 4 dpf. (F) mRFP-Actr10 expression in actr10 mutant neurons suppressed axon terminal swellings and mitochondrial accumulation. Yellow arrowhead points to the region of the axon terminal expressing mRFP-Actr10. Note that mRFP-Actr10 negative axon terminals (red arrowhead) display axonal swelling and high levels of Cytochrome c labeling. Lines in D'', E'' and F'' indicate regions used for line scan analysis. (G) Line scans show that mRFP-Actr10 expression rescues Cytochrome c levels in actr10 mutant axons (compare pink/red and yellow lines). Pigment cells (*) obscure some nerves. Scale bars = 10 µm.