FIGURE

Fig. S5

ID

ZDB-FIG-170428-24

Publication

Eldred et al., 2017 - Self-organising aggregates of zebrafish retinal cells for investigating mechanisms of neural lamination

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Fig. S5

M�ller Glia are absent in aggregates treated with 25?M DAPT.

Zebrafish retinal cells were cultured using the GFAP:GFP transgenic line which strongly labels M�ller Glia (but is also expressed in undifferentiated cells at low levels). Aggregates were treated with either DMSO or 25?M DAPT from the equivalent of 45hpf onwards. (A-B) Aggregates treated with DMSO as a control. (A) GFAP:GFP expressing MG can be seen throughout the aggregate, extending axonal like projections into the aggregate (filled arrow). (B) DAPI. (C-D) Aggregates treated with 25?M DAPT. (C) GFAP:GFP can only be seen at low levels, and no axonal projections can be seen indicating a lack of MG present. (D) DAPI. Scale bar = 10?m.

Expression Data

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Antibody Labeling

Phenotype Data

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