FIGURE

Fig. 2

ID

ZDB-FIG-170315-8

Publication

Zhang et al., 2015 - G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition

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Fig. 2

Gpr183 plays an indispensable role during definitive hematopoiesis in zebrafish. (A) Generation of gpr183 mutant using the CRISPR/Cas9 technique. PAM sequence is 'TGG' (in blue); alignment of WT and mutated sequences is listed below. (B, C) Reduced expression of cmyb (or runx1) and rag1 in gpr183 mutant embryos (B) and clotrimazole-treated embryos (C). Red arrowheads indicate cmyb or runx1 expression in AGM, and white circles denote rag1 expression in the thymus. (D) The number of HE in Tg(gpr183^−/−;flk1:mCherry/runx1:en-GFP) embryos is attenuated significantly compared with that in control embryos. White arrowheads denote HE in the AGM region. (E) Dot-plot quantification analysis of HE in sibling control and gpr183 mutant. Error bar represents SD. ^****P < 0.0001. (F) Hematoxylin-eosin staining of head kidney section showing hematopoietic defect in 12-week-old gpr183 heterozygous mutant fish.

Expression Data

Genes:	GFP mCherry myb rag1 runx1
Fish:	AB/TU TU gpr183a^ioz9/ioz9 (AB/TU) ioz1Tg; s896Tg gpr183a^ioz9/ioz9; ioz1Tg; s896Tg
Condition:	chemical treatment: clotrimazole
Anatomical Terms:	angioblastic mesenchymal cell thymus ventral wall of dorsal aorta
Stage Range:	Prim-25 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU gpr183a^ioz9/ioz9 (AB/TU) gpr183a^ioz9/ioz9; ioz1Tg; s896Tg
Condition:	chemical treatment: clotrimazole
Observed In:	angioblastic mesenchymal cell definitive hemopoiesis head kidney head kidney cell thymus ventral wall of dorsal aorta ventral wall of dorsal aorta angioblastic mesenchymal cell
Stage Range:	Prim-25 to Days 45-89

Phenotype Detail

Acknowledgments

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