FIGURE

Fig. 5

ID

ZDB-FIG-170206-20

Publication

Lu et al., 2017 - Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish

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Fig. 5

Tbx20 overexpression rescues the cardiac defects present in tbx20 mutant embryos. (A) Diagram of the inducible activity of the tbx20-GR (glucocorticoid receptor) fusion protein. The addition of DXS (dexamethasone) is required for the translocation of the tbx20-GR protein into the nucleus. (B-D) Dorsal views of myl7 expression at the 16-somite stage in wild type embryos (B), tbx20 mutants (C) and tbx20 mutants injected with tbx20-GR mRNA (D). The cardiomyocyte production defects in tbx20 mutant embryos are substantially rescued by the injection of tbx20-GR mRNA. (E) Schematic representation of the hsp70l:Tbx20 transgene used for tbx20 overexpression. (F-H) Dorsal views of myl7 expression at the 16-somite stage in wild type (F), tbx20 mutant (G) and Tbx20^-/-; Tg(hsp70l:Tbx20) embryos (H). (I-K) Frontal views of myl7 expression at 48hpf in wild type (I), tbx20 mutant (J) and Tbx20^-/-;Tg(hsp70l:Tbx20) embryos (K). Overexpression of Tbx20 successfully restored cardiomyocyte production in tbx20 mutant embryos.

Expression Data

Gene:	myl7
Fish:	WT tbx20^{la1110/la1110}
Anatomical Terms:	anterior lateral plate mesoderm heart primordium cardiac muscle cell heart
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx20^{la1110/la1110}
Observed In:	anterior lateral plate mesoderm heart primordium heart
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(2), Lu, F., Langenbacher, A., Chen, J.N., Tbx20 drives cardiac progenitor formation and cardiomyocyte proliferation in zebrafish, 139-148, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.