Fig. S6

Cell autonomous requirement for scfd1 in neural crest cells for cartilage differentiation. (A-D) Sox10: eGFP transgene expression in control and scfd1 mutant embryo. A and C, lateral views with anterior to left. B and D, ventral views with anterior to the top. (E) Schematic diagram of the transplantation approach. At 4 hpf, cells from a donor (wild type or mutant) Sox10:eGFP transgenic embryo are transplanted to the animal pole of a wild type or scfd1 mutant host embryo. (F, J and N) At 24 hpf, donorderived neural crest cell migration to the oral ectoderm is evident regardless of donor genotype. Lateral views with anterior to the top. (G-I) At 72hpf, in control transplantation experiments, donor cells were observed contributing to cartilage. (J-M) Conversely, in none of the transplants where scfd1 mutant donor cells were used was contribution to cartilage noted. (N-P) We also observed that wild type donor GFP-positive neural crest cells in scfd1 mutant host embryos formed cartilage. (Q-T) At 72 hpf, cartilage staining reveals wild type donor-derived cells partially rescuing anterior neurocranium defects in scfd1 mutant embryos. Red arrowheads indicate cartilage derived from wild type donor cells.