Fig. 4

DM treatment induces loss of M cartilage and edn1 expression in wdr68^{hi3812/hi3812} zebrafish.

(A-D) Ventral views of 5dpf Alcian stained craniofacial cartilages of zebrafish raised at 24°C and treated with DMSO or 10μM DM at 14–15 somites stage. A) Wildtype embryo treated with DMSO control. B) wdr68^{hi3812/hi3812} mutant treated with DMSO show joint fusions between M and PQ. C) Wildtype embryo treated with DM reveal no defects in craniofacial cartilages. D) wdr68^{hi3812/hi3812} mutants treated with DM show severe reduction in PQ and deletion of M. E) DM-treated mutants show significantly more severe defects compared to the control group (p<0.040). (F-I) Dorsal views of edn1 ISH analysis on 20 somites stage embryos treated with DMSO or 10μM DM starting at the 14–15 somites stage. F) Wildtype embryos treated with DMSO control. G) wdr68^{hi3812/hi3812} mutants treated with DMSO control show reduced edn1 expression. H) Wildtype embryos treated with DM show mildly reduced edn1 expression. I) wdr68^{hi3812/hi3812} mutants treated with DM lack edn1 expression.