FIGURE

Fig. 6

ID

ZDB-FIG-161222-17

Publication

Lau et al., 2016 - Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation

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Fig. 6

Gonad development at 29 dpf in the control (cyp19a1a^+/−; fish A–D) and mutant (cyp19a1a^−/−; fish E–L). In the control fish, the gonads (boxed in the photo) with much enhanced GFP signal (GFP++; A and B) contained large LPOs and little amount of stromal tissues, indicating the development towards the true ovary whereas those with weak or reduced GFP signal (GFP+; C and D) had few POs but large number of apoptotic cells (arrowheads), suggesting the development towards males. In the mutant fish, all the mutant individuals showed weak GFP signal comparable with that of the control males. Most mutant gonads (G–L) contained undifferentiated germ cells with some undergoing apoptosis (arrowheads). A few contained OPs (e.g., fish E) but with large amount of stromal tissues.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp19a1a^umo5/umo5; zf45Tg/+ (AB)
Observed In:	female gonad development
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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