Fig. 2

Tissue-Restricted Abnormalities in gemin5 Mutants, Related to Figure S5

(A) Normal numbers and distribution of fluorescent lymphoid progenitor cells visualized in an ikaros:eGFP transgenic background (arrows). Scale bar, 10 μm.

(B) Reduced numbers of ikaros-expressing lymphocyte progenitors in the mutant thymus. Scale bar, 25 μm.

(C) Diagnostic whole-mount RNA in situ hybridization pattern in gemin5 mutants using rag1 (thymus encircled in purple), and gh (hypophysis encircled in orange) at 5 dpf. Scale bar, 100 μm.

(D) Lack of thymocytes in the thymus (red circle) at 8 dpf (H&E staining). Scale bar, 25 μm.

(E) Expression of the thymic epithelial marker foxn1 (thymus encircled in purple) at 5 dpf. Scale bar, 100 μm.

(F) Normal receptive capacity of mutant thymic epithelium for whole kidney marrow hematopoietic cells isolated from adult wild-type ikaros:eGFP transgenic fish. At 5 dpf, the number of transplanted cells per embryo was determined. The overall success rate of the transplantation procedure is identical between the genotypes.

(G) Distribution of ikaros-expressing neurons in the mutant hindbrain. Scale bar, 100 μm.

(H) Retinal cell layers (1, ganglion cell layer; 2, inner plexiform layer; 3, inner nuclear layer; 4, outer plexiform layer; 5, outer nuclear layer; 6, photoreceptor cell layer; 7, retinal pigment epithelium) (right panel); H&E staining. Scale bar, 25 μm.

(I) Body size at 8 dpf. Scale bar, 500 μm.

No differences were observed for gemin5^+/+ and gemin5^+/− fish for any of the analyzed parameters.