FIGURE

Fig. 3

ID

ZDB-FIG-161219-30

Publication

Bremer et al., 2016 - Myosin phosphatase Fine-tunes Zebrafish Motoneuron Position during Axonogenesis

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Fig. 3

Mypt1 is required in the environment to restrict excessive axon branching.

(A, B) SV2 antibody labeling at 25 hpf reveals disorganized motor axon morphologies in mypt1^p82emcf mutants (B) compared to wildtype siblings (A). Unlike wildtype CaP motoneurons (C), individually labeled mutant motor axons (using mnx1:mKate) project with frequent changes in projection direction (dots), and exhibit excessive branching (arrowheads). The gray dashed line marks the border of the spinal cord. (E, F) Transplantation of rhodamine-dextran labeled wildtype blastula stage cells into mypt1 mutants analyzed at 26–27 hpf exhibit mutant motor axon phenotypes (E). Conversely, transplantation of mutant motoneurons into a wildtype host results in wildtype-like motor axon projections (F).

Expression Data

Gene:	mKate2
Antibody:	Ab-SV2
Fish:	WT ppp1r12a^{p82emcf/p82emcf} p188Tg ppp1r12a^{p82emcf/p82emcf}; p188Tg
Anatomical Terms:	CaP motoneuron CaP motoneuron axon
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ppp1r12a^{p82emcf/p82emcf} ppp1r12a^{p82emcf/p82emcf}; p188Tg
Observed In:	CaP motoneuron axon CaP motoneuron axonogenesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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