Fig. 4

Hair bundle density in the zebrafish saccule. (A) Schematic of the saccular epithelium showing the location of our five sampling regions along the rostral-caudal axis. The confocal image on the right is an example of a phalloidin-labeled saccule. All boxes are 50 × 50 μm and are drawn to scale. V = ventral, R = rostral. (B) Comparison of bundle density in *AB fish and transgenic lines on a *AB background. Hair bundle density significantly differs by genotype and saccular location (2-way ANOVA, F_5,225 = 6.88, p < 0.001; and F_4,225 = 79.06, p < 0.001, respectively). Bonferroni-corrected posthoc tests were then used to compare each transgenic line to the *AB wildtype fish, where **p < 0.01 indicates significant differences from *AB at that saccular location. (C) There is no significant difference in saccular hair bundle density between wildtype strains (2-way ANOVA, F_2,96 = 2.65, p = 0.07). (D) Saccular bundle density does not differ between transgenic ET4 fish and wildtype TL fish (2-way ANOVA, F_1,31 = 0.08, p = 0.78). Data are presented as mean ± S.E. N = 4–12 fish per group.