FIGURE

Fig. S4

ID

ZDB-FIG-161130-9

Publication

Grainger et al., 2016 - Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells

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Fig. S4

Proliferation in the aorta.

kdrl:mCherry; cmyb:GFP fish were injected with 10mM EdU at 26 hpf and examined by confocal microscopy for GFP+;mCherry+ cells that had proliferated (EdU+, purple) by 32 hpf (A. n=10) or 30 hpf (B. n=10). Open arrows indicate nascent HSPCs, while closed arrows indicate HSPCs that are undergoing EHT. FACS histograms in control (C) and Wnt9a morphant (D) fish sorted for G1 (mCherry+) and endothelium (GFP+). Note the increase in endothelial cells in the G1 fraction of wnt9a morphants. Scale bars= 30µm. Error bars represent standard deviation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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