FIGURE

Fig. 1

ID

ZDB-FIG-161110-8

Publication

Cheng et al., 2016 - Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish

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Fig. 1

GFP is highly expressed in the ventricle of v12 zebrafish embryos. (A-D′) GFP is not detected in 75% epiboly embryos (A,A′), and is initially expressed in the somite boundaries at ~24 hpf (B). Cardiac GFP expression starts at ~35 hpf (C,C′) and GFP is highly expressed in the ventricle at 50 hpf (D,D'). Arrows indicate ventricle. (E-H) Ventricular GFP expression in v12 heterozygotes (E-F) and v12 homozygotes (G-H) at 84 hpf shown at high magnification. Arrows indicate ventricle. (I-I'') Immunostaining shows that β-catenin and GFP colocalize close to the cell membrane in v12 heterozygous hearts at 78 hpf. (J-J'') Immunostaining shows that GFP is absent from Z-disc (α-actinin) in v12 heterozygous hearts at 78 hpf. Scale bars: 100 µm in A-D,E-H; 20 µm in I; 5 µm in J.

Expression Data

Gene:	EGFP
Antibodies:	Ab1-actn Ab14-ctnnb
Fish:	vclb^gd4Gt/gd4Gt vclb^gd4Gt/+ vclb^gd4Gt
Anatomical Terms:	cardiac ventricle heart membrane heart Z disc heart tube somite border
Stage Range:	75%-epiboly to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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