FIGURE

Fig. 4

ID

ZDB-FIG-161101-4

Publication

Poon et al., 2016 - Development of the cardiac conduction system in zebrafish

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Fig. 4

Loss of endocardium and hemodynamic stimulation affects development of the CCS. A: 3 dpf CCS:clo sibling. B: 3 dpf CCS:clo^m3^9-^/^- mutant. SAN CCS cells (arrows) spread widely in clo^m3^9-^/^-. C, E: Control CCS embryos at 2 dpf and 3 dpf, respectively. D, F: tnnt2 morphants at 2 dpf and 3 dpf, respectively. E′, F′: The same embryos as in E and F, showing dsRed expression of CCS:Tg(myl7:dsRed). Dotted white lines outline the chambers of the embryonic zebrafish heart. A-D: lateral view; E-H: ventral view. Abbreviations: A, atrium; SAN, sino-atrial region; V, ventricle.

Expression Data

Genes:	DsRed2 EGFP
Fish:	sqet33mi28Et sqet33mi59BEt f2Tg; sqet33mi28Et f2Tg; sqet33mi59BEt f2Tg; sqet33mi28Et + MO1-tnnt2a f2Tg; sqet33mi59BEt + MO1-tnnt2a npas4l^s5/s5; sqet33mi28Et npas4l^s5/s5; sqet33mi59BEt
Knockdown Reagent:	MO1-tnnt2a
Anatomical Terms:	myocardium sinoatrial node sinoatrial ring
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2Tg; sqet33mi28Et + MO1-tnnt2a f2Tg; sqet33mi59BEt + MO1-tnnt2a npas4l^s5/s5; sqet33mi28Et npas4l^s5/s5; sqet33mi59BEt
Knockdown Reagent:	MO1-tnnt2a
Observed In:	cardiac conduction system sinoatrial node
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns