FIGURE

Fig. 7

ID

ZDB-FIG-161028-27

Publication

Warga et al., 2016 - Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development

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Fig. 7

The ogre nervous system lacks later born neurons. (A, B) Expression of: (A) notch1b in proliferating cells and (B) dlA in neuroblasts. (C, D) High magnification view of neuroblasts visualized with dlA and DAPI. Numbers beside circled dlA-positive cells indicate their number of nuclei or if the cell was apoptotic (apop), white bar shows the boundary between somites 3 and 4. (E) Expression of islet1 in primary motoneurons (pmn) and Rohon-Beard sensory neurons (rb). Inset (E′) expression of col2a in floorplate neurons (flp) and hypochord cells (hc). (F) Expression of: pax2a and egr2b in CoSA interneurons (CoSA) and inset (F′) high magnification of CoSA interneurons. Numbers beside cells indicate their number of nuclei. (G) Quantification of multinucleate neuroblasts between somites 2 and 8; n indicates the number of embryos at different stages. Differences are significant compared between 14 h and 17 h (p<0.001) and compared between 17 h and 21.5 h (p<0.05; contingency tables/Pearson′s chi square).

Expression Data

Genes:	dla egr2b isl1a notch1b pax2a
Fish:	WT racgap1^ta52a/ta52a
Anatomical Terms:	CoSA neuroblast primary motor neuron proliferative region rhombomere 3 rhombomere 5 Rohon-Beard neuron spinal cord interneuron
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	racgap1^ta52a/ta52a
Observed In:	brain CoSA neuroblast primary motor neuron proliferative region Rohon-Beard neuron spinal cord commissural neuron specification spinal cord interneuron spinal cord motor neuron differentiation
Stage Range:	5-9 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 418(2), Warga, R.M., Wicklund, A., Richards, S.E., Kane, D.A., Progressive loss of RacGAP1/ogre activity has sequential effects on cytokinesis and zebrafish development, 307-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.