FIGURE

Fig. 4

ID

ZDB-FIG-161017-4

Publication

Hosseini et al., 2016 - Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis

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Fig. 4

Ultrastructure of correlated macrophages and neutrophils in 3D serial block-face SEM images. (A) High-resolution CLSM image of an infected tail fin after live-cell imaging and fixation. (B) SBFSEM image of the same region as in A showing correlated macrophages (arrowheads) and neutrophils (arrows). (C) Higher magnification SBFSEM images of the two macrophages and two neutrophils indicated in A and B. (D) Frames from time-lapse imaging showing the macrophages and neutrophils in C at previous time points. The macrophage (M1) with a fragmented nucleus contains a high Mm content, indicated by the asterisk in C. This macrophage phagocytized the bacterial content ~30min before fixation. Macrophage M2 contains a large aggregate of Mm which was phagocytized Mm ~1.5h before fixation. Neutrophil N1 shows a rapid signal disappearance ~45min before the fixation (D). Neutrophil N2 was recruited to the bacterial aggregate ~30min before fixation (D). Scale bars: 10µm (A-C); 2µm (D).

Expression Data

Genes:	DsRed2 EGFP
Fish:	gl22Tg; nz50Tg
Condition:	bacterial treatment by injection: Mycobacterium marinum
Anatomical Terms:	macrophage neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.