FIGURE

Fig. S3

ID

ZDB-FIG-160921-13

Publication

Ryckebüsch et al., 2016 - Tmem2 regulates cell-matrix interactions that are essential for muscle fiber attachment

Other Figures

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Fig. S3

Disruption of fast muscle fiber attachment in MZtmem2 mutants. (A-D) Immunofluorescence reveals muscle fiber organization, using phalloidin (red in A,B) to recognize both fast and slow fibers, F59 (green in A,B) to recognize slow fibers (Devoto et al., 1996), and F310 (green in C,D) to recognize fast fibers (Nord et al., 2014); lateral views, dorsal up, at 26 hpf. (A,B) In addition to exhibiting detachment of F59+ slow muscle fibers (B); see also Fig. 1H), MZtmem2 mutants display detachment of F59- fast muscle fibers (arrows, B′), in contrast to the attached fibers observed in Mtmem2 siblings (A). (C,D) Similarly, MZtmem2 mutants (D) display detachment of F310+ fast muscle fibers (arrow, D2), in contrast to the attachment seen in Mtmem2 siblings (C). C′ and D′ show closer views of regions outlined by white rectangles in C and D.

Expression Data

Antibody:	Ab-F310
Fish:	cemip2^sk38/sk38 cemip2^sk38/+
Anatomical Term:	fast muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cemip2^sk38/sk38
Observed In:	fast muscle cell trunk musculature trunk musculature muscle tendon junction
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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