FIGURE

Fig. S3

ID

ZDB-FIG-160830-7

Publication

Just et al., 2016 - The mediator complex subunit Med10 regulates heart valve formation in zebrafish by controlling Tbx2b-mediated Has2 expression and cardiac jelly formation

Other Figures

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Fig. S3

Figure S3: Ectopic expression of Med10 rescues png mutant embryos, whereas knockdown of zmed10 phenocopies the png mutant phenotype. (A-D) Ectopic expression of wild-type med10 RNA can rescue the heart phenotype of a significant proportion of png mutant embryos (84.5 ± 4.2%, n = 85, 3 independent experiments) (B, C), whereas KCl control-injection has no effect (A, C). (D) Hematoxylin/Eosin staining of sagittal histological sections of a med10 RNA-injected png mutant heart at 72 hpf. Endocardial cushion development (black arrow) in png mutant hearts is reconstituted. (E-G) Knockdown of zmed10 by injection of Morpholino-modified antisense oligonucleotides (MO-med10) phenocopies the png mutant phenotype (MO1-med10: 98.37% ± 0.45%, n = 240, 3 independent experiments; MO2-med10: 95.53% ± 0.68%, n = 185, 3 independent experiments) (E, G), whereas injection of the same amount of standard control Morpholino-modified antisense oligonucleotides (MO-control) does not impact on AV canal development (F, G).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-med10 WT + MO3-med10
Knockdown Reagents:	MO1-med10 MO3-med10
Observed In:	atrioventricular ring endocardial cushion
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.