FIGURE

Fig. 3

ID

ZDB-FIG-160817-9

Publication

Kimelman, 2016 - A novel cold-sensitive mutant of ntla reveals temporal roles of Brachyury in zebrafish

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Fig. 3

Analysis of gene expression in ntla mutant embryos. A-J: Wild-type, ntla^-/-, and ntla^cs/cs embryos were placed at 17°C at shield stage, then incubated at 29°C the next morning (when the embryos were at bud stage) and raised to the 14-somite stage. Whereas ntla^-/- embryos had essentially no expression of the presomitic genes tbx16 and tbx6l or the notochordal gene col9a2, ntla^cs/cs, embryos had strong expression of all three genes. All these embryos were hybridized with the indicated probe at the same time and for the same length of time. K-P: In contrast, when ntla^cs/cs embryos were kept continuously at 17°C, expression of all three genes was considerably diminished. Arrows show accumulation of col9a2 in the posterior of ntla^cs/cs embryos.

Expression Data

Genes:	col9a2 tbx16
Fish:	WT tbxta^b195/b195 tbxta^w181/w181
Condition:	cold exposure
Anatomical Terms:	notochord segmental plate
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbxta^b195/b195 tbxta^w181/w181
Condition:	cold exposure
Observed In:	notochord segmental plate
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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