FIGURE

Fig. 4

ID

ZDB-FIG-160802-13

Publication

Tang et al., 2016 - Gene knockout of nuclear progesterone receptor provides insights into the regulation of ovulation by LH signaling in zebrafish

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Fig. 4

Phenotype analysis of the npr^+/+ and npr^-/- female zebrafish at 75 dpf.

(a) Gross morphology of the npr^+/+ and npr^-/- fish. (b,c) Body length (b) and body weight (c) of npr^+/+ and npr^-/- mutants (n = 6). (d) Ovary morphology of the npr^+/+ and npr^-/- fish. (e), Microscopic examination of follicles from npr^+/+ and npr^-/- mutants. Follicles were collected at 15:00 pm and matured oocytes (red arrows) could be observed in the npr^-/- mutant. (f) The gonado somatic index (GSI) of npr^+/+ and npr^-/- fish (n = 6). (g) Spawning assay. No npr^-/- fish succeeded in spawning (n = 30). (h) Ovary histology of the npr^+/+ and npr^-/- fish. The outer layer cells of the matured follicles (indicated by arrow) failed to breakdown in the npr^-/- fish. (i) Oocyte maturation assay. FG stage follicles from npr^+/+ and npr^-/- fish were treated with IGF3 or 17α, 20β-DHP, and the percentages of follicles that underwent germinal vesicle breakdown (GVBD) were recorded. Each value represents the mean value ± SEM (n = 4) of three independent experiments (*P < 0.05; ***P < 0.001 vs npr^+/+).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pgr^zf723/zf723 (AB)
Observed In:	embryo development ovarian follicle development ovary ovulation trunk whole organism
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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