FIGURE

Fig. 2

ID

ZDB-FIG-160713-35

Publication

Hoffman et al., 2016 - Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

cntnap2ab Mutants Display Nighttime Hyperactivity
(A) Experimental setup (Prober et al., 2006 and Rihel et al., 2010).
(B) Locomotor activity of cntnap2a^Δ25/Δ25cntnap2b^Δ7/Δ7 (cntnap2ab, red) and wild-type (WT; blue) sibling-matched larvae over 72 hr. Hyperactivity in mutants worsens on successive nights (arrows). The magnified activity profile on night 6 is shown.
(C) Average locomotor activity of cntnap2ab versus wild-type. p = 0.00012 (one-way ANOVA, comparing all genotypes on all nights); p = 0.0193, 0.0236, and 0.0073, nights 4, 5, and 6, respectively.
(D) Hierarchical clustering of the cntnap2ab behavioral fingerprint (red arrow) compared with the fingerprints of wild-type larvae exposed to a panel of 550 psychoactive agents from 4–6 dpf ( Rihel et al., 2010). Each rectangle in the clustergram represents the Z score, or the average value in SDs relative to the behavioral profiles of wild-type exposed to DMSO alone (magenta, higher than DMSO; cyan, lower than DMSO). The cntnap2ab profile correlates with agents that induce nighttime arousal (“Correlating Drugs”).
(E) A magnified section of the clustergram, highlighting compounds that correlate with the cntnap2ab mutant behavioral fingerprint.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cntnap2a^ya2188/+; cntnap2b^ya2043/+
Observed In:	circadian sleep/wake cycle, sleep regulation of circadian sleep/wake cycle, sleep whole organism
Stage Range:	Day 4 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron