FIGURE

Fig. 3

ID

ZDB-FIG-160608-3

Publication

Wang et al., 2016 - Knockdown of zebrafish Nanog increases proliferation of primordial germ cells during early embryonic development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The expression of zNanog in adult zebrafish tissues. (I) Western blot analysis of the His-tagged zNanog fusion protein probed with the anti-His antibody, anti-zNanog antibody or pre-immune serum, respectively. (II) Western blot analysis of zNanog in zebrafish tissues. The zNanog protein was expressed in the ovary, heart, testis, and liver, as detected by a single band of 55 kDa, but not in the muscle or brain. Pre-immune serum was used as a negative control. (III) The expression of zNanog in the zebrafish ovary and testis. The localization of zNanog in the ovary and testis cryosections was detected by immunofluorescence analysis (A-F: ovary; G-L: testis). A, D, G, and J: DAPI staining; B and H: zNanog polyclonal antibody and the secondary green fluorescent antibody; E and K: pre-immune serum and the secondary green fluorescent antibody; C, F, I, and L: Merge. I: stage I oocyte, II: stage II oocyte, III: stage III oocyte, IV: stage IV oocyte, SG: spermatogonia, SC: spermatocytes. Scale bars: 0.5 mm in all panels.

Expression Data

Gene:	nanog
Antibody:	AB1-nanog
Fish:	AB
Anatomical Terms:	heart liver oocyte stage I cytoplasm oocyte stage II cytoplasm oocyte stage III ovary spermatogonium testis
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Growth Diff.