FIGURE

Fig. 3

ID

ZDB-FIG-160525-27

Publication

Zhao et al., 2016 - An essential role for Grk2 in Hedgehog signalling downstream of Smoothened

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Fig. 3

Grk2 acts upstream of Gli processing

A. Prox1a and Eng expression in the myotome of 30hpf wildtype (WT) and MZgrk2 embryos injected with mRNAs encoding Shh, dnPKA or mSmoA1GFP. Each panel shows Prox1a in green, Eng in red and the merged images with DAPI staining in blue. The colabelling for Prox1a and Eng (orange) is indicative of MP differentiation (n = 30 for each sample). Scale bar, 50 µm.

B. Western blot showing levels of Gli2aFL and Gli2aR forms in 20hpf wildtype (WT) and MZgrk2 (MZ) embryos injected with mRNA encoding Shh, dnPKA or mSmoA1GFP, respectively. γ-tubulin was used as a loading control. Three biological replicates of this analysis were performed.

Expression Data

Antibody:	Ab1-eng
Fish:	WT grk3^i283/i283
Anatomical Terms:	myotome fast muscle cell myotome muscle pioneer
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	grk3^i283/i283
Observed In:	myotome fast muscle cell myotome muscle pioneer slow muscle cell
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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