Fig. 2 S2
Aplnr activation enhances Nodal target gene expression. (A-I) Expression of the canonical Nodal target genes gsc, flh and sox32 in WT (A-C), aplnrb RNA injected embryos (D-F) and embryos treated with the Aplnr agonist ML233 (G-I) at 8 hpf. Embryos are viewed from the dorsal side with anterior to the top. (J-O) Expression of the canonical Nodal target genes gsc, flh and sox32 in aplnra/b morphant embryos with or without ML233 at 8 hpf. Embryos are viewed from the dorsal side with anterior to the top. (P-U) Expression of the canonical Nodal target genes gsc, flh and sox32 in oep morphant embryos with or without the injection of aplnrb RNA and addition of ML233 at 8 hpf. Embryos are viewed from the dorsal side with anterior to the top. |