FIGURE

Fig. S6

ID

ZDB-FIG-160518-14

Publication

Eden et al., 2016 - Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression

Other Figures

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Fig. S6

(a left panel) RT -PCR analysis in zebrafish hearts from Myoscape knockdown morphants compared to controls showed a successful splice site mutation as indicated by a clear double band. (a right panel) Western blot analysis of zebrafish hearts from Myoscape knockdown morphants compared to controls, revealing a significant (>60%) reduction of Myoscape protein content after morpholino injection. (b+d) Histological (HE staining, scale bar 100µm), immunohistochemical (scale bar 100µm) (c) and ultrastructural evaluation of zebrafish heart (e) sections and skeletal muscle tissue (f) showing no gross architectural deficits after Myoscape ablation, indicating no obvious role of Myoscape in development or cellular sub-architecture of these tissues in zebrafish (scale bar EM 1 µm).

Expression Data

Gene:	strip2
Antibodies:	Ab-MF20 Ab-S46
Fish:	WT WT + MO1-strip2
Knockdown Reagent:	MO1-strip2
Anatomical Terms:	atrial myocardium ventricular myocardium whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-strip2
Knockdown Reagent:	MO1-strip2
Observed In:	cardiac atrium morphogenesis cardiac muscle cell differentiation cardiac ventricle morphogenesis myocardium sarcoplasmic reticulum myocardium T-tubule myocardium Z disc whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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