FIGURE

Fig. 2

ID

ZDB-FIG-160517-2

Publication

Banote et al., 2016 - β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner

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Fig. 2

Knockdown of Appb inhibit Mauthner cell development. Whole-mount immunostaining of embryos at 48 hpf (A-C). Confocal images (anterior to the top) of hindbrain stained with anti-neurofilament RMO44 antibody marking reticulospinal neurons in control MO (A) and appb MO injected (B,B2) embryos. Note the 1-cell/no-cell phenotype in morphants. The large M-cells (M, in A) are located in r4. The absent M-cell is noted by an asterisk (B,B′). M-cell number is rescued in embryos coinjected with appb MO and appb mRNA (C). Cell counts of M-cell number (D). M, Mauthner cell; n, number of embryos and r3-r6, rhombomeres 3-6. Scale bar, 50 µm.

Expression Data

Antibody:	Ab1-neurofilament-m
Fish:	WT WT + MO5-appb
Knockdown Reagent:	MO5-appb
Anatomical Terms:	Mauthner neuron axon Mauthner neuron cell body reticulospinal neuron axon reticulospinal neuron cell body
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-appb
Knockdown Reagent:	MO5-appb
Observed In:	hindbrain hindbrain neuron differentiation Mauthner neuron
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 413(1), Banote, R.K., Edling, M., Eliassen, F., Kettunen, P., Zetterberg, H., Abramsson, A., β-Amyloid precursor protein-b is essential for Mauthner cell development in the zebrafish in a Notch-dependent manner, 26-38, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.