ZFIN Figure: Ando et al., 2016, Fig. 1

Fig. 1

Generation of Tg zebrafish lines for live imaging of MCs. (A) A schematic structure of the BAC clone (CH1073-606I16) used to generate Tg zebrafish lines for live imaging of MCs. cDNA encoding either EGFP, mCherry or Gal4FF was inserted at the start codon of pdgfrb gene. (B) Confocal stack fluorescence image of trunk vasculature in a 96hpf TgBAC(pdgfrb:Gal4FF);Tg(UAS:GFP);Tg(fli1a:Myr-mCherry) larva. Lateral view, anterior to the left. Merged image of pdgfrb:Gal4FF;UAS:GFP (green) and fli1a:Myr-mCherry (red). (C-F) Confocal images of hindbrain vasculature (C,D), hyaloid vessels (E) and anterior region of dorsal aorta (F) in the TgBAC(pdgfrb:EGFP);Tg(fli1a:Myr-mCherry) larvae at 60hpf (C) and 80hpf (D-F). Dorsal view, anterior to the left. Merged images of pdgfrb:EGFP (green) and fli1a:Myr-mCherry (red). In C, the boxed areas are enlarged to the right. (G) Confocal images of trunk vasculature in a 1mpf TgBAC(pdgfrb:EGFP);Tg(fli1a:Myr-mCherry) juvenile. Cross-sectional views (200µm thick) through the caudal region as depicted in Fig. S1H are shown. Upper left, pdgfrb:EGFP (green); upper center, fli1a:Myr-mCherry (red); upper right, merged image. The boxed areas labeled a and b are enlarged below. (H) Confocal images of blood vessels in the intercostal muscle of a 1mpf TgBAC(pdgfrb:EGFP);Tg(fli1a:Myr-mCherry) juvenile. Pleural tissue as indicated by the box shown in g was cut out and immunostained with anti-α-SMA antibody to visualize VSMCs. The merged image of pdgfrb:EGFP (green) and fli1a:Myr-mCherry (red) is shown on the left (a). The boxed area in a is enlarged to the right: pdgfrb:EGFP (b), fli1a:Myr-mCherry (c), α-SMA (d), merge of pdgfrb:EGFP (green) and fli1a:Myr-mCherry (red) (e) and merge of pdgfrb:EGFP (green), fli1a:Myr-mCherry (red) and α-SMA (blue) (f). (g) Brightfield image of the thorax showing the region where the image shown in a was taken. BA, basilar artery; BCA, basal communicating artery; CCtA, cerebellar central artery; DA, dorsal aorta; LDA, lateral DA; HV, hyaloid vessel; PCS, posterior communicating segment; PHBC, primordial hindbrain channel. Scale bars: 20µm (enlarged images in C and H; D-F); 50µm (B,C); 100µm (G,H).

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab6-act
Fish:	ncv1Tg; ncv22Tg ncv1Tg; ncv24Tg; nkuasgfp1aTg
Anatomical Terms:	basal communicating artery endothelial cell basal communicating artery vascular associated smooth muscle cell basilar artery endothelial cell basilar artery vascular associated smooth muscle cell blood vessel blood vessel vascular associated smooth muscle cell capillary capillary pericyte cerebellar central artery endothelial cell cerebellar central artery vascular associated smooth muscle cell dorsal aorta endothelial cell dorsal aorta vascular associated smooth muscle cell dorsal longitudinal anastomotic vessel endothelial cell dorsal longitudinal anastomotic vessel vascular associated smooth muscle cell hyaloid vessel endothelial cell hyaloid vessel vascular associated smooth muscle cell intersegmental vessel endothelial cell intersegmental vessel vascular associated smooth muscle cell lateral dorsal aorta endothelial cell lateral dorsal aorta vascular associated smooth muscle cell posterior communicating artery endothelial cell posterior communicating artery vascular associated smooth muscle cell primordial hindbrain channel endothelial cell primordial hindbrain channel vascular associated smooth muscle cell segmental intercostal artery segmental intercostal artery vascular associated smooth muscle cell trunk vasculature trunk vasculature pericyte trunk vasculature vascular associated smooth muscle cell
Stage Range:	Pec-fin to Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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