FIGURE

Fig. 2

ID

ZDB-FIG-160513-6

Publication

Nepal et al., 2016 - Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs

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Fig. 2

Expression pattern revealed by in situ hybridization of mature miR-9 and pri-miRNA transcripts during prim6 stage. (A) Expression pattern of mature miR-9 is mostly restricted to telencephalon. Left panels represent lateral views and right panels represent dorsal views. (B-I) Expression pattern of miR-9 pri-miRNA transcripts is also restricted to telencephalon with differences in the spatiotemporal details among different pri-miRNAs, such as: miR-9-2 (developing retina, white asterisk; Figure 2C), miR-9-4 (posterior brain regions, white arrowhead; Figure 2E) and miR-9-6 (hypothalamus, white arrow; Figure 2H). (J) Amplified region of 5′ RACE PCR product (corresponding to probe-1) of miR-9-5 maps exactly to the alternative intronic CAGE tags. (K) Unique discrete amplification band obtained from 5′ RACE PCR products obtained for miR-9-5 (with intronic CAGE tags). Multiple non-specific bands and/or a smear were obtained for miR-9-4 that did not have alternative intronic CAGE tags.

Expression Data

Genes:	dre-mir-9-1 mir9-2 mir9-3 mir9-4 mir9-5 mir9-6 mir9-7
Fish:	WT
Anatomical Terms:	brain posterior region hypothalamus optic cup telencephalon
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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