FIGURE

Fig. 7

ID

ZDB-FIG-160321-9

Publication

Banerjee et al., 2016 - Dysregulation of Wnt-signaling and a candidate set of miRNAs underlie the effect of metformin on neural crest cell development

Other Figures

Fig. 7

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Fig. 7

Metformin inhibits NC development in zebrafish. Control embryos (no metformin treatment, A, C, I, K), embryo with 25 mM metformin treatment (B, D, L), and embryos with 15 mM metformin treatment (J) were fixed at stages indicated in lower left. Expression of snai2 (A, B), sox9b (C, D), mitfa (I, J), and coexpression of emx3, krox20, and myod (K, L) were analyzed by in situ hybridization. Embryos from sox10:eGFP transgenic line were analyzed for reporter gene expression at 15-somite stage with 25 mM metformin treatment (F, F inset) and no treatment (E, E inset). Skin pigmentation at 120 hours in control embryos (G) and in embryos treated with 15 mM metformin (H, as indicated). Scale bar = 100 µm, (A-F) dorsal views, anterior to top, (G-L) lateral views anterior to the left; s, somite; ov, otic vesicle. All data are representative of at least three independent experiments.

Expression Data

Genes:	EGFP egr2b emx3 mitfa myod1 snai2 sox9b
Fish:	WT ba2Tg
Condition:	chemical treatment: metformin
Anatomical Terms:	anatomical structure melanocyte neural crest
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT ba2Tg
Condition:	chemical treatment: metformin
Observed In:	melanocyte neural crest neural crest cell development pigmentation
Stage Range:	5-9 somites to Day 5

Phenotype Detail

Acknowledgments

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