FIGURE

Fig. 7

ID

ZDB-FIG-160317-8

Publication

Lisse et al., 2016 - Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing

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Fig. 7

(a) IKK is necessary for H₂O₂-induced HEK01 keratinocyte migration after wounding. Scratch assays were performed with H₂O₂ (0.1 µM) and Wedelolactone (50 µM) using HEK01 cells. Two-way ANOVA at an alpha = 0.05 (95% confidence interval) and Bonferroni’s multiple comparison post-tests were utilized. Significance is denoted with asterisks: *p < 0.05, **p < 0.01 (n ≥ 3-5 cell culture experiments). (b) Rapid H₂O₂ production using 1 µM HPF (hydrogen peroxide fluorogenic probe) at the scratch (sc) margin of HEK01 keratinocytes within 30 minutes. Bar = 100 µm (c) Rapid subcellular accumulation of IKKα within injured HEK01 cells at the scratch (sc) margin (white arrows) after 30 minutes compared to unscratched cells. Orthogonal views (red arrows) of an injured cell show peri-nuclear and cytoplasmic distribution and accumulation of IKKα. Bar = 20 µm. (d) Schema of overall findings.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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