FIGURE

Fig. 6

ID

ZDB-FIG-160314-5

Publication

Tulotta et al., 2016 - Inhibition of signaling between human CXCR4 and zebrafish ligands by the small molecule IT1t impairs the formation of triple-negative breast cancer early metastases in a zebrafish xenograft model

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

The CXCR4 antagonist IT1t reduces metastatic tumor burden in vivo. IT1t (20µM) was applied into the cell medium for 24h prior to engraftment in zebrafish embryos to antagonize CXCR4 receptor activation (A). The percentage of live cells after treatment was not significantly different than in the control condition (three independent experiments) (B). The metabolic activity, readout of cell growth, was not significantly affected when increasing concentrations of IT1t were used (C). Pre-treatment in vitro caused a reduction in tumor burden at the secondary site in vivo, both at 2dpi (D) and 4dpi (E). Cancer cell burden increased over time from 2 to 4dpi in the control group, whereas it remained at comparable levels upon treatment (F). Data set in F is obtained by using the same data points as shown in D and E. Number of larvae is n=64 (DMSO) and n=75 (IT1t) at 2dpi and n=59 (DMSO) and n=56 (IT1t) at 4dpi. (G) Effect of CXCR4 inhibition on tumor burden over time is shown. Scale bars: 50µm. Data are mean±s.e.m. from two independent experiments. Statistical analysis: two-tailed, unpaired t-test and ANOVA with Bonferroni post-hoc test for datasets with two or more groups respectively. ****P<0.0001; **P=0.005; ns, P>0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.