FIGURE

Fig. S3

ID

ZDB-FIG-160309-18

Publication

Tang et al., 2016 - Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish

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Fig. S3

Emergence of clonal dominance among three similar TALL clones.

(a) Donor animals engrafted with monoclonal T-ALL arising in the CG1 background. (b-c) Monoclonal T-ALLs were implanted into syngeneic CG1 strain fish and assessed for LPC frequency by limiting dilution cell transplantation (b) or latency of regrowth (c). T-ALLs have similar LPC frequency and latency, with p-values noted on each panel. (d-e) Confocal imaging of engrafted rag2^E450fs (casper) fish at 15 dpt (d) and 21 dpt (e). White arrow denotes site of injection and imaging. (f-g) Relative proportions of each fluorescent clone contained within individual engrafted animals quantified by fluorescence area within confocal images (f) and manual cell counts (g). Scale bars equal 5 mm in whole animal images and 50 µm in confocal images.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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