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Fig. 5

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ZDB-FIG-160304-13

Publication

Diotel et al., 2016 - Mapping of Brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation

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Fig. 5

Quantification of Blbp and cyp19a1b-GFP (AroB) expression in proliferative radial glial cells in different neurogenic regions. A: Blbp (blue) and PCNA (red) staining on cyp19a1b-GFP (green) transgenic zebrafish line in the dorsomedian telencephalon. Proliferative cells (PCNA) can be Blbp-positive (inset 1), GFP-positive (inset 2), Blbp/GFP-positive (inset 3) or Blbp/GFP-negative (inset 4). The double negative group has been previously proposed to correspond to further committed precursors suggested to be neuroblasts ( März et al., 2010). B: Columns 1, 2, 3 and 4 corresponds to the respective insets in A for each channel. In 1, asterisks point to a proliferative cells displaying Blbp staining but not GFP. In 2, asterisks highlight a proliferative cells displaying GFP staining but not Blbp. In 3, asterisks show a proliferative cell displaying both GFP and Blbp staining, and neither staining in 4. C-G: In each region of interest, proliferative (PCNA) radial glial cells were checked for the expression of Blbp and cyp19a1b-GFP (AroB). Counting was performed on 6 adult female zebrafish (n = 6), on 325 cells for the Vv, 270 for the Vd, 205 for the A, 158 for the PPv, 185 for the Hc Div and 205 for the nucleus of the LR. The selected regions correspond to the zebrafish brain atlas ( Wullimann et al., 1996) section level number 71 for the Vv and Vd; 131 for the A; 168 for the PPv and the Hd LR. Statistical analysis was performed using one-way ANOVA. Error bars correspond to standard errors (n = 6 zebrafish). * 0,01 < p < 0,05; ** 0,01 < p < 0,0001; ***p < 0,0001.

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Acknowledgments

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Reprinted from Gene expression patterns : GEP, 20(1), Diotel, N., Vaillant, C., Kah, O., Pellegrini, E., Mapping of Brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation, 42-54, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns