FIGURE

Fig. 3

ID

ZDB-FIG-160226-60

Publication

Kao et al., 2015 - Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation

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Fig. 3

Early cardiomyocyte specification does not appear reduced in pbx4 mutant embryos. (A–F) RNA in situ expression at the 10 s stage of cardiomyocyte specification genes (A–B) gata4, (C–D) gata5, and (E–F) hand2 in the anterior lateral plate mesoderm (ALPM) in (A,C,E) control and (B,D,F) pbx4^b557-/- embryos. Expression of the Pbx-dependent gene krox20, marking hindbrain rhombomeres 3 and 5 (r3, r5 in A), was included to distinguish between control and pbx4^b557-/- embryos [12,19]. Arrows indicate ALPM expression domains in control embryos. The junction between hand2-expressing ALPM and posterior lateral plate mesoderm domains is noted by arrowheads (E). For (A–D), n e 10 for each marker in pbx4^b557-/- embryos and n e 30 for each marker in control embryos, all with similar expression patterns. For (E–F), pbx4^b557-/- embryos display expanded hand2 expression (n = 14; 13/14 with expanded expression) compared to control embryos (n = 47; all similar). Embryos are shown in dorsal view, anterior towards the left.

Expression Data

Genes:	egr2b gata4 gata5 hand2
Fish:	AB pbx4^b557/b557 (AB)
Anatomical Terms:	anterior lateral plate mesoderm lateral plate mesoderm posterior lateral plate mesoderm rhombomere 3 rhombomere 5
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pbx4^b557/b557 (AB)
Observed In:	lateral plate mesoderm
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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