FIGURE

Fig. 7

ID

ZDB-FIG-160217-6

Publication

Ge et al., 2014 - Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction

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Fig. 7

Long-range animally-directed transport is not affected in hecate mutants.

(A-C) Paths of injected 0.2 um fluorescent beads after injection into the vegetal pole with a single (A,B) or double (C) injection in wild-type (A,C) or hec mutant (B) embryos. (A′-C′) show merged imaged including the fluorescent channel (shown in A-C) and corresponding DIC optics at low intensity. The extent and frequency of bead transport appeared similar in wild-type and mutant embryos (A,B, see text). Injections into two opposite sides of the vegetal pole results in multiple animally-directed paths, indicating that the entirety of the mediolateral cortex is competent for bead movement. Arrowheads and arrows in (A-C) indicate site of injection in the vegetal region and animally-directed paths along mediolateral regions, respectively. Magnification bar in (C′) corresponds to 100 µm for all panels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	grip2a^p06ucal grip2a^p08ajug grip2a^t2800
Observed In:	cytosolic transport
Stage:	1k-cell

Phenotype Detail

Acknowledgments

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