FIGURE

Fig. 3

ID

ZDB-FIG-160210-8

Publication

Dempsey et al., 2015 - Determination of the source of SHG verniers in zebrafish skeletal muscle

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

SHG verniers seem to cross intact membrane boundaries, indicating their illusory origin.

These panels display a single ‘xz’ cross section within a trunk somite of a fixed 5 dpf zebrafish larva, where the membranes and nuclei are FP labeled (see Supporting Information, Methods). (A) Verniers (‘Y’-shaped) are visible within much of the thick filament-derived SHG signal (magenta). (B) Fluorescent reporter expression (green) illuminates the plasma membranes (diffuse borders) of the several visible muscle fibers as well as the nuclei within one particular cell (arrows). (C) The merged image of fluorescence and SHG signal shows that many SHG verniers seem to cross the physical membrane boundaries of the muscle cells (boxed regions, compare to the same boxed regions in panel A,B). Sarcomeric components should not connect across adjacent cell boundaries under normal physiological circumstances, especially in an unperturbed WT larva. Scale bar: 10 µm.

Expression Data

Gene:	Citrine
Fish:	tpm3^ct5aGt
Anatomical Terms:	muscle cell nucleus muscle cell plasma membrane
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.