FIGURE

Fig. 3

ID

ZDB-FIG-160210-3

Publication

Wong et al., 2015 - Production of reproductively sterile fish by a non-transgenic gene silencing technology

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Fig. 3

Zebrafish dnd-MO-Vivo induced sterility in zebrafish.

Embryos initially treated with 60 or 40 µM of dnd-MO-Vivo developed into male-like adults. (A) No difference in appearance or overall size was observed between treated adult fish and control males. (B) No significant difference in body-weight (Mean ± SD) of 3-month-old fish (N = 12 by random sampling) was noted among dnd-MO-Vivo treated fish and control males (Data that share the same letter are not significantly different from each other). Examination of gonadal tissue showing (C) a fully-developed testis of a control male fish, (D) a fully-developed ovary of a control female fish, (E) the gonads of dnd-MO-Vivo treated fish that developed into a thin filament-like tissue. Photomicrographs (F–H) show (F) advanced spermatogenesis in the testis of a control male fish, (G) a well-developed ovary of a control female fish with oocytes at advanced stages of gametogenesis, (H) the gonad of dnd-MO-Vivo treated fish appears to be under-developed and surrounded with a large amount of adipocytes, without advanced gonadal structure or germ cells. Scale bar: white = 200 µm, black = 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pu5Tg + MO1-dnd1
Knockdown Reagent:	MO1-dnd1
Observed In:	female sex differentiation male sex differentiation sperm testis whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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