Fig. 1

Amiodarone causes the ectopic expression of cdh5 through its repression of snail1b expression.

(A-H) WISH of snail1a (A, B) snail1b (C, D), snail2 (E, F) and snail3 (G, H) in indicated embryos during 72 hpf. Control embryos were treated with DMSO. 72 hpf zebrafish embryos treated with 15 µM Amiodarone from 55 to 72 hpf. (A-H) Lateral view of 72 hpf embryos. (A′ and H′) Ventral view of 72 hpf embryos which focus on the heart region. WISH revealed that snail1a, snail2 and snail3 were not detected at the AVC, while snail1b is detected at the AVC (C′ red arrow). Incubation of 15 µM Amiodarone from 55 to 72 hpf did not influence snail1a or snail1b at head region, but the expression of snail1b at AVC was lost (D′ red arrow). (I) WISH of cdh5 in control-MO or snail1b-MO-injected embryos. (J) Western blot analysis of Cdh5 protein level in control-MO (Ctrl), Amiodarone-treated embryos (15A), snail1b-MO-injected embryos, snail1b-mRNA-injected embryos, and Amiodarone-treated plus snail1b-mRNA-injected embryos. V: ventricle, A: atrium. The number of embryos displaying similar pattern was indicated in each figure. The relative intensities of each protein were as indicated. The α-tubulin was used as an internal control.