FIGURE

Fig. 1

ID

ZDB-FIG-160205-127

Publication

Zhang et al., 2015 - The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish

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Fig. 1

Anterior migration of PCT and morphogenesis of the neck segment are impaired in taz morphants. (A) In situ hybridization of embryos with the PCT-specific probe, slc20a1a. Note the absence of anterior migration and convolution of PCT in taz morphants. (B) Neck region morphogenesis is arrested in taz morphants. In situ hybridization using pax2a probe (red), followed by anti-BrdU staining (green) to visualize the neck region morphologies and detect proliferating cells in the neck regions in control embryos (a, c, e, g, i) and taz morphants (b, d, f, h, j). Note in f, the neck regions remained aligned with the A–P axis in the taz morphant, rather than repositioned to align with the mediolateral axis as in the normal embryo (e). (g, h) Individual optical sections of stacks. (i, j) Projected images of stacks. Anterior is to the top. Bars, 20µm.

Expression Data

Genes:	pax2a slc20a1a
Fish:	WT WT + MO1-wwtr1 + MO4-tp53
Knockdown Reagents:	MO1-wwtr1 MO4-tp53
Anatomical Terms:	pronephric proximal convoluted tubule pronephric tubule
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wwtr1 + MO4-tp53
Knockdown Reagents:	MO1-wwtr1 MO4-tp53
Observed In:	pronephric proximal convoluted tubule pronephric proximal tubule development pronephric tubule whole organism anterior-posterior axis
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M., The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish, 328-35, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.