FIGURE

Fig. 2

ID

ZDB-FIG-160205-1

Publication

Beauchemin et al., 2015 - Dynamic microRNA-101a and Fosab expression controls zebrafish heart regeneration

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Fig. 2

Depletion of miR-101a expression promotes cardiomyocyte proliferation in the injured apex. (A-D) Control or Tg(hs:miR-101a-sp) animals were injured and subjected to daily heat treatment. Hearts were collected at 3dpa for histology, cryosectioned at 10µm and stained with antibodies to detect Mef2 (green) and Pcna (red) to identify proliferating cardiomyocytes (CMs) (arrowheads) at the injured apex (A,C) and lateral wall (remote zone) (B,D). Insets in A,C are higher magnifications of the areas within dashed boxes. (E) CM proliferation indices were determined by representing Mef+Pcna+ cells as a percentage of total Mef2+ cells. Depletion of miR-101a expression doubled CM proliferation indices at 3dpa in the injured apex but not in the remote zone. (F) qPCR studies show ~80% reduction of miR-101a expression levels in Tg(hs:miR-101a-sp) ventricles (blue line) compared with the control group (red dashed line) under conditions of heat treatment. n=5-7; *P<0.05 (Student′s t-test); error bars represent s.e.m. HT, heat treatment.

Expression Data

Antibodies:	Ab1-mef2 Ab2-pcna
Fish:	EKW mdi1Tg (EKW)
Conditions:	heat shock, resection: cardiac ventricle resection: cardiac ventricle
Anatomical Terms:	cardiac muscle cell regenerating tissue cardiac muscle cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mdi1Tg (EKW)
Condition:	heat shock, resection: cardiac ventricle
Observed In:	regenerating tissue cardiac muscle cell proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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