FIGURE

Fig. 2

ID

ZDB-FIG-151215-11

Publication

Li et al., 2015 - Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development

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Fig. 2

Morpholino knockdown of nr2f1b causes defects in vascular development. a In uninjected control embryos, the arota (da) and posterior cardinal vein (pcv) have formed by 30 hpf and intersegmental vessels (isv) have reached the DLAV at the dorsal aspect of the embryo. At the same stage ISVs are stalled mid-somite in nr2f1b ^i1e2 (b), nr2f1b ^e1i1 (e) and nr2f1b ^ATG (f) morphants. Overexpression of nr2f1b has no obvious defect in vasculature (d), but rescues the defect of ISV stalling (solid arrowhead) as shown in (c). g Quantification of percentage of completed ISV shows a 40 % increase compare to nr2f1b morphants (*** refers to p < 0.0001 by an unpaired student′s t-test. Scale bars are 50 µm for (a-f). h and i Imaging of endothelial nuclei in green and vessels in red at 30 hpf in wild-type control and nr2f1b MO-treated embryos using Tg(fli1a:nEGFP) ^y7 :(kdrl:mCherry) ^ci5 double transgenic line. nr2f1b morphants showed reduced ISV nuclei numbers (i). j Quantification of ISV nuclei number in nr2f1b morphants (n = 18) compared to wild-type control (n = 16). ***P < 0.001, Student t test.

Expression Data

Gene:	GFP
Fish:	la116Tg la116Tg + MO4-nr2f1b
Knockdown Reagent:	MO4-nr2f1b
Anatomical Term:	posterior cardinal vein
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO2-nr2f1b la116Tg + MO3-nr2f1b la116Tg + MO4-nr2f1b ci5Tg; y7Tg + MO4-nr2f1b
Knockdown Reagents:	MO2-nr2f1b MO3-nr2f1b MO4-nr2f1b
Observed In:	intersegmental vessel intersegmental vessel cell posterior cardinal vein vasculature development
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.