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Fig. 3

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ZDB-FIG-151214-24

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Diao et al., 2015 - PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart

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Fig. 3

Efficient siRNA knockdown of aldh1a2 and dusp6 in the adult zebrafish heart. (A, B) Immunostaining showed Aldh1a2 expression in the epicardium and endocardium of negative control (siN.C.)-injected hearts (A, A′) and siAldh1a2 siRNA-injected hearts (B, B′). MF20 staining marks the myocardium. (C, D) Western blots showing that nanoparticle-encapsulated siAldh1a2 reduced Aldh1a2 expression to a level comparable to that in the sham group, compared to the nanoparticle-encapsulated siN.C. and naked siAldh1a2 groups at 2 dpa (C), and quantitative knockdown of Aldh1a2 (D). (E, F) Immunostaining showed Dusp6 expression in the epicardium of siN.C.-injected control hearts (E, E′) and siDusp6-injected hearts (F, F′). Mef2C staining marks the myocardium. (G, H) Western blots showing that nanoparticle-encapsulated siDusp6 reduced Dusp6 expression to a level comparable to that in the sham group, compared with the nanoparticle-encapsulated siN.C. and naked siDusp6 groups at 2 dpa (G), and quantitative knockdown of Dusp6 (H). Scale bars, 100 µm.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 406(2), Diao, J., Wang, H., Chang, N., Zhou, X.H., Zhu, X., Wang, J., Xiong, J.W., PEG-PLA Nanoparticles facilitate siRNA knockdown in adult zebrafish heart, 196-202, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.