FIGURE

Fig. S1

ID

ZDB-FIG-151214-15

Publication

Gokey et al., 2015 - The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish

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Fig. S1

Expression of the zebrafish V-ATPase accessory protein Atp6ap1b. (A) RT-PCR of temporal expression of atp6ap1b and atp6ap1a through the first three days of development. β-actin was used as a loading control. (B) Schematic diagram and alignment of human, mouse and zebrafish Atp6ap1 proteins. (C) RT-PCR detected maternal mRNA (at the 1-cell stage) of several V-ATPase subunits and then sustained expression through 2dpf. β-actin mRNA was used as a loading control. (D) Homozygous atp6ap1ba82/a82 mutants developed pigmentation phenotypes relative to wild-type siblings. (E) Western blot analysis of Atp6ap1 in wild-type and atp6ap1ba82/a82 mutant embryos at 3, 5 and 7 dpf. A prominent band just under 50 kDa was reduced in mutants. Tubulin was used as a loading control. The graph depicts relative intensities of the Atp6ap1 band from one representative experiment. (F) Fluorescent immunostaining of Atp6ap1 in surface cells of wild-type and atp6ap1ba82/a82 mutant embryos at 3, 5 and 7 dpf. The graph shows the average fluorescent intensity of Atp6ap1 staining.

Expression Data

Genes:	atp6ap1a atp6ap1b atp6v0ca atp6v0d1 atp6v1f atp6v1g1
Antibody:	Ab1-atp6ap1
Fish:	WT atp6ap1b^au82/au82
Anatomical Terms:	cell plasma membrane whole organism
Stage Range:	1-cell to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp6ap1b^au82/au82
Observed In:	cell plasma membrane whole organism
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 407(1), Gokey, J.J., Dasgupta, A., Amack, J.D., The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish, 115-30, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.