FIGURE

Fig. 5

ID

ZDB-FIG-151203-13

Publication

Voelz et al., 2015 - A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides

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Fig. 5

A zebrafish larval swim bladder infection model for mucormycosis. (A) AB wild-type zebrafish larvae were injected in the swim bladder 4days post-fertilization (d.p.f.) with a LD or a HD of M. circinelloides NRRL3631. (B) No significant mortality was observed after mucormycete spore injection into the swim bladder 4d.p.f. (PVP versus LD Mc P=0.172; PVP versus HD Mc P=0.403; LD Mc versus HD Mc P=0.561; n=68). (C) Spores remained viable within the zebrafish larvae over the time course of 48h.p.i. (D,E) Macrophages and neutrophils are recruited to the site of infection after injection of viable spores into the swim bladder. Injection of UV-killed spores did not induce significant phagocyte recruitment. (F,G) No significant induction of the pro-inflammatory cytokines il1b or tnfα was detected at 6 or 24h.p.i. with the exception of weak tnfα induction at 24h.p.i.

Expression Data

Gene:	tnfa
Fish:	AB
Condition:	fungal treatment by injection: Mucor circinelloides
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB i114Tg c264Tg; gl25Tg
Condition:	fungal treatment by injection: Mucor circinelloides
Observed In:	macrophage neutrophil swim bladder whole organism
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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