FIGURE

Fig. 1

ID

ZDB-FIG-150824-11

Publication

Helker et al., 2015 - The hormonal peptide Elabela guides angioblasts to the midline during vasculogenesis

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Fig. 1

Angioblast migration to the midline is not regulated by Vegf.

(A–F) Schematic (A) and confocal (B–F) in vivo time-lapse imaging of angioblasts (green) and their migration in a Tg(fli1a:EGFP)^y1 embryo, injected with H2B-mCherry mRNA (purple, all nuclei), dorsal views at indicated time points. Arrows indicate the initial migration to the midline, and the coalescing into the dorsal aorta (DA). (G–J) Loss of Vegf-signaling components by vegfaa/vegfab double morpholino (MO) injection (H) or in vegf receptor 2 (kdrl) mutants (J) does not affect angioblast migration. Confocal projections of Tg(fli1a:EGFP)^y1 embryos in dorsal views at 17 hpf. For each condition, n > 15.

Expression Data

Gene:	EGFP
Fish:	y1Tg kdrl^{hu5088/hu5088}; y1Tg y1Tg + MO1-vegfab + MO3-vegfaa
Knockdown Reagents:	MO1-vegfab MO3-vegfaa
Anatomical Terms:	angioblastic mesenchymal cell blood vessel endothelial cell vascular cord
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kdrl^{hu5088/hu5088}; y1Tg y1Tg + MO1-vegfab + MO3-vegfaa
Knockdown Reagents:	MO1-vegfab MO3-vegfaa
Observed In:	angioblast cell migration from lateral mesoderm to midline
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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