FIGURE

Fig. 3

ID

ZDB-FIG-150804-18

Publication

Strate et al., 2015 - Glypican4 promotes cardiac specification and differentiation by attenuating canonical Wnt and Bmp signaling

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Fig. 3

Compromised cardiomyocyte differentiation in kny/gpc4 mutants. (A,B) Embryonic hearts of wild-type and Tg(nkx2.5BAC:galFF/UAS:h2a-gfp, myl7:dsred, kny^+/) embryos after immunohistochemistry against GFP (Nkx2.5, green), DsRed (Myl7, red) and Amhc (white) at 48hpf. Boxed areas indicate the regions shown in higher magnification in E3,F3 and used for quantification in C,D. (C) Numbers of nkx2.5⁺ and myl7⁺ cells in the ventricles of wild-type siblings and kny/gpc4 mutants (n=5). (D) Ratio of nkx2.5⁺/myl7⁺ cells in wild-type siblings and kny/gpc4 mutants (n=5). (E-E3) Higher magnification views of the ventricle of a wild-type sibling, showing sparse nkx2.5⁺-only cells (white asterisks). (F-F3) Corresponding area in a kny/gpc4 mutant, displaying a typical cluster of nkx2.5⁺ cells. Scale bars: 15µm. Results are represented as mean±s.e.m. **Pd0.01, ***Pd0.001 in paired t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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