FIGURE

Fig. 4

ID

ZDB-FIG-150609-10

Publication

Love et al., 2015 - Rest represses maturation within migrating facial branchiomotor neurons

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Fig. 4

Morphological analysis reveals precocious maturation of Rest-deficient FBMNs. (A–G) Analysis of FBMNs at 20 and 28 hpf in Tg(zCREST1:membRFP) wild type embryos (A and B) and MZrest^sbu29/sbu29 embryos (C and D). Average measurements from 5 embryos are shown for wild type and MZrest^sbu29/sbu29 (E–G) embryos at each stage. The wild type length-to-width ratio decreases from 20 to 28 hpf, as neurons become rounder; by contrast MZrest^sbu29/sbu29 FBMNs are round at both 20 and 28 hpf (E). Both number and length of protrusions increase over time in wild type and MZrest^sbu29/sbu29 embryos (F and G). MZrest^sbu29/sbu29 FBMNs have significantly more and longer protrusions than wild type FBMNs. (H–J) Volume measurements of FBMNs at 22 hpf. Whole cell and nuclear volume were measured for 10 “immature” neurons in r4 (yellow dots) versus 10 “mature” neurons in r6 (red dots) for 5 wild type (H) and 5 MZrest^sbu29/sbu29 (I) embryos. Wild type FBMN whole cell volume increases significantly as neurons migrate from r4 to r6; MZrest^sbu29/sbu29 FBMN whole cell volume does not alter as neurons migrate from r4 to r6 and is similar to that of wild type neurons in r6. Nuclear volume does not change significantly. Mean±SEM; ^*P<0.05; ^***P<0.001, ^****P<0.0001, n.s.=not significant.

Expression Data

Gene:	RFP
Fish:	ch100Tg rest^sbu29/sbu29; ch100Tg
Anatomical Terms:	facial nerve motor nucleus membrane facial nerve motor nucleus motor neuron
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rest^sbu29/sbu29; ch100Tg
Observed In:	facial nerve motor nucleus facial nerve motor nucleus motor neuron facial nerve motor nucleus neuron maturation facial nerve motor nucleus neuron projection motor neuron neuron projection rhombomere 4 facial nerve motor nucleus rhombomere 5 facial nerve motor nucleus rhombomere 6 facial nerve motor nucleus
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 401(2), Love, C.E., Prince, V.E., Rest represses maturation within migrating facial branchiomotor neurons, 220-35, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.