FIGURE

Fig. 6

ID

ZDB-FIG-150527-13

Publication

Kyöstilä et al., 2015 - A Missense Change in the ATG4D Gene Links Aberrant Autophagy to a Neurodegenerative Vacuolar Storage Disease

Other Figures

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Fig. 6

Immunostaining with (A-C) anti-Pvalb7 and (D-F) anti-zebrinII antibody show loss of cerebellar Purkinje cells in atg4da morphants. (A,D) Control embryos at 4.5 dpf. (B,E) Morphants with mild phenotype show partial loss of cerebellar Purkinje cells. (C,F) Morphants with strong phenotype show either total loss of Purkinje cells or presence of few differentiated neurons, which are laterally located in the cerebellum. (G-I) Labeling of cerebellar granule cells with anti-Vglut1 antibody in control and morphant embryos. (H) Mildly affected morphants show reduced expression of Vglut1 in the cerebellum. (I) Vglut1 expression is strongly reduced in embryos showing severe phenotype. White arrowheads indicate the region of cerebellum. Abbreviations: hb, hindbrain.

Expression Data

Genes:	pvalb7 slc17a7a
Antibodies:	Ab1-aldoc Ab1-pvalb7 Ab1-slc17a7
Fish:	AB AB + MO1-atg4da
Knockdown Reagent:	MO1-atg4da
Anatomical Terms:	cerebellar granule cell Purkinje cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-atg4da
Knockdown Reagent:	MO1-atg4da
Observed In:	cerebellar granule cell cerebellar neuron development Purkinje cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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