FIGURE

Fig. 6

ID

ZDB-FIG-150507-5

Publication

Benini et al., 2015 - slc7a6os Gene Plays a Critical Role in Defined Areas of the Developing CNS in Zebrafish

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Fig. 6

Analysis of pax2a expression in slc7a6os morphant embryos.

Control and slc7a6os morphant embryos were analyzed by WISH for pax2a gene expression at different developmental stages. At 16 hpf expression of pax2a is down regulated in morphants: in particular a strong down-regulation for this marker was observed in the midbrain-hindbrain boundary (B, lateral view and E, dorsal view). No evident alterations were observed for otic vesicles and optic stalk in slc7a6os injected embryos compared to controls. At 28 hpf the defect in midbrain-hindbrain boundary is still clearly evident in morphants (H, flat mounted embryos). The midbrain-hindbrain boundary alteration is also evident in the embryo lateral view (M). Rescue experiments with synthetic slc7a6os mRNA confirmed the specificity of the phenotype observed (C, F, I, N). Abbreviations: MHB, midbrain–hindbrain boundary; op, optic stalk; ov, otic vesicles; t, telencephalon.

Expression Data

Gene:	pax2a
Fish:	AB AB + MO1-slc7a6os
Knockdown Reagent:	MO1-slc7a6os
Anatomical Terms:	midbrain hindbrain boundary optic stalk otic vesicle telencephalon
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-slc7a6os
Knockdown Reagent:	MO1-slc7a6os
Observed In:	midbrain hindbrain boundary
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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