FIGURE

Fig. 6

ID

ZDB-FIG-150422-55

Publication

George et al., 2015 - An early requirement for nkx2.5 Ensures first and Second heart field ventricular identity and cardiac function into adulthood

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Rescue of nkx2.5^-/-;nkx2.7^-/- embryos validates a dose-dependent role of nkx2.5 and suggests a unique function for nkx2.7. Frontal views, anterior to the top, of MF20/S46 immunofluorescence (as in Fig. 3) at 52 hpf. In contrast to nkx2.5^-/-;nkx2.7^-/- offspring of a single Tg(hsp70l:nkx2.5-EGFP) carrier following heat shock at one time point (Fig. 5L-O), a transgenic nkx2.5^-/-;nkx2.7^-/-embryo subject to heat at 11 somites and 21 somites demonstrates enhanced rescue of ventricular and atrial size and identity defects (A). Despite only moderate improvement in offspring from a cross of two transgenic parents at 11 somites (B) and 21 somites (C), performing heat shock at two time points to augment Tg(hsp70l:nkx2.5-EGFP) expression further yields normalization of cardiac chamber morphology (D). Yet, residual, ectopic S46⁺ cardiomyocytes highlight chamber identity abnormalities in the late-differentiating SHF-derived population (D; arrow).

Expression Data

Antibodies:	Ab-MF20 Ab-S46
Fish:	nkx2.5^vu179/vu179; nkx2.7^vu413/vu413; fcu1Tg
Condition:	heat shock
Anatomical Terms:	atrial myocardium myocardium ventricular myocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 400(1), George, V., Colombo, S., Targoff, K.L., An early requirement for nkx2.5 Ensures first and Second heart field ventricular identity and cardiac function into adulthood, 10-22, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.