Fig. 1

Loss of sin1 function leads to embryonic axis enlargement.

A-B. At the dose used in this analysis, sin1 morpholino efficiently blocks translation of sin1 RNAs. A control morpholino (A) or a morpholino targeting the translation initiation site of sin1 (B) was co-injected at the 1-cell stage with mRNAs encoding a Sin1-mCherry fusion. Both images were acquired with the same exposure time. C-P. In situ hybridisations of genes expressed in the neuroectoderm (otx2), the endoderm (sox17), the mesoderm (ta) or the ventro-lateral mesoderm (tbx6). Embryos are either un-injected, injected with a control morpholino or with sin1 morpholino. They were fixed at mid-gastrulation. The three germ layers appear correctly formed. Mesoderm stainings nevertheless reveal a lateral widening of axial structures, quantified in L and P.